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Available ordering format
Liquid
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Immunogen
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3-™-dimentrylaminopropyl) carbodiimide
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG1 (clone G104)
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C for one year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
Plant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
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Possible reactivity
antibody is not species specific
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
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References
(WB): Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP): Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF): Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
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Scientific context
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
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Notes
protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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