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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana nitrate transporter NRT1.4, Q9SZY4, At2g26690
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), western blot (WB)
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Connected products
collection of antibodies to other proteins localized in tonoplastPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
63.5 | 70 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Raphanus sativus
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Possible reactivity
Arabidopsis thaliana, Raphanus sativus
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
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References
to be added when available
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Scientific context
Nitrate transporter functions in transporter activity in response to jasmonic acid stimulus and response to wounding. This protein is coded by ntp2 gene.
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.This protein is present in plant tissues in very low levels.Protocol for isolation of plant vacuolar membranes can be found here.
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Protein number
Q9SZY4
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TAIR number
AT2G26690
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