NAB1, nucleic acid binding protein 1, Chlamydomonas

• Catalog number
  AS08 333
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Lyophilized
• Immunogen
  His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunoprecipitation (IP)
• Connected products
  Collection of antibodies to Chlamydomonas proteins
• Recommended dilutions for use
  1: 10 000 standard ECL (WB)
• Molecular weight expected аpparent
  26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)
• Verified reactivity
  Chlamydomonas reinhardtii
• Possible reactivity
  Chlamydomonas reinhardtii
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  to be added when available
• References
  Craft et al. (2015). Tubulin transport by IFT is upregulated during ciliary growth by a cilium-autonomous mechanism. J Cell Biol. 2015 Jan 19;208(2):223-37. doi: 10.1083/jcb.201409036. Epub 2015 Jan 12. Desai et al. (2014). Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes. Cytoskeleton (Hoboken). 2014 Dec 31. doi: 10.1002/cm.21206.Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421
• Scientific context
  In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).
• Notes
  NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.
• Protein number
  Q8GV23
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  NAB1   nucleic   acid   binding   protein   Chlamydomonas  
• Gene symbol
  NAB1
• Short name
  NAB1, nucleic acid binding protein 1, Chlamydomonas
• Alternative name
  NAB1, nucleic acid binding protein 1, Chlamydomonas

Gene info

• Identity
  HGNC:7626
• Gene
  NAB1
• Long gene name
  NGFI-A binding protein 1
• Synonyms gene name
  • NGFI-A binding protein 1 (EGR1 binding protein 1)
• Synonyms name
  • EGR1 binding protein 1
• Locus
  2q32.2
• Discovery year
  1996-10-11
• Entrez gene record
  4664
• Pubmed identfication
  • 7624335
  • 8668170
  • 9418898
• RefSeq identity
  • NM_005966
• VEGA ID
  OTTHUMG00000132689

MeSH Data

• Name
  Blood Grouping and Crossmatching
• Concept
  Scope note: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
• Tree numbers
  • E01.370.225.625.120
  • E01.370.225.812.385.120
  • E05.200.625.120
  • E05.200.812.385.120
  • E05.478.594.385.120
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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