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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Hordeum vulgare MT2a sequence, UniProt I6NQG8
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS13 2684 | MT1a | Metallothioneine type1, rabbit antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB) on recombinant protein, 1: 400 (IL)
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Molecular weight expected аpparent
7.7 kDa
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Verified reactivity
Horderum vulgare
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Possible reactivity
Horderum vulgare
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
This antibody has so far worked on recombiant protein in western blot. Immunolocalization experiment is described in detail in Schiller et al. (2013).
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References
Schiller et al. (2013). Barley metallothioneins differ in ontogenetic pattern and response to metals. Plan Cell Environ June 28.
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Scientific context
MT2a (Metallothionein 2a) is a cysteine-rich, low molecular mass protein with a metal ion binding function. Metallothionenins (MTs) are involved in many physiological processes such as metal ion homeostasis, detoxification of excess metals and protection against oxidative stress.
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Notes
None
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Protein number
I6NQG8
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TAIR number
Refer to NCBI
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