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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from available MnSOD sequences in di and monocotyl plants including Arabidopsis thaliana O81235, At3g10920
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxide dismutaseAS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutaseAS07 219 | anti-CCS | chloroplastic copper chaperone for SODPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 2000 - 1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
25 | 25 kDa
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Verified reactivity
Arabidopsis thaliana , Armeria maritima, Brassica napus, Iris pumila, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Pisum sativum, Solanum tuberosum
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Possible reactivity
Gossipium mexicanum, Hordeum vulgare, Picea sitchensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Triticum aestivum, Vitis vinifera, Zea mays
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19 Dmitrovic et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64. Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6. Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29. Momcilovic et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
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Scientific context
Plant MnSOD catalyzes the dismutation of superoxide to molecular oxygen and water. One subunit of the homotetramer complex binds 1 Mn cofactor. Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD. Synonymes: MSD1, SODA, SOD3
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Notes
Freshly prepared reducing agent like DTT needs to be used in a sample buffer. Otherwise MnSOD will migrate at 50 kDa.
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Protein number
O81235
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TAIR number
AT3G10920
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