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Available ordering format
Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
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Immunogen
Synthetic 5-Methyohytryptamine-glutaric anhydride-conjugated to protein carrier-BSA.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 50 µl of sterile water and 50 µl of glycerol
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Storage condition
store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
indirect ELISA (ELISA) with conjugated 5-Methoxytryptamine glutaric anhydride
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Connected products
AS09 597 | anti-melatonin rat antibodiesSecondary antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
232.278 g/mol
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Verified reactivity
conjugated melatonin
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Possible reactivity
conjugated melatonin
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
for example of immunocytochemistry protocol check heretotal IgG was purified by ammonium sulfate precipitation
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References
to be added when available
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Scientific context
Melatonin - N-acetyl-5-methoxytryptamine, is a natural compund involved in circadian rhythms in animals and in plants. In plants it is involved in regulation of photoperiod, defence responses and acts as a scavenger of reactive oxygen species.
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Notes
Specificity has been determined using ELISA competition experiments using conjugate of glutathione-glutaraldehyde protein.CompoundsCross-reactivity ratio (a) 5-Methoxytryptamine-GA-BSA15-Hydroxytryptamine-GA-BSA1/ >7 500DL 5-Methoxytryptophan-GA-BSA1/ >9 500Tryptamine-GA-BSA1/ >50 0005-Methoxytryptamine-G-BSA1/ >50 000(a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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