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Available ordering format
Lyophilized
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Immunogen
Recombinant full length mCherry expressed and purified from E. coli.
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG2a
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Purification
Purified from tissue culture supernatant.
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Immunocytochemistry (IC), Western Blot (WB)
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Connected products
AS15 2987 | GFP (affinity purified), rabbit antibodyAS15 2999 | GFP (total IgG), rabbit antibodyAS15 3000 | GFP (protein A purified) rabbit antibodyAS15 2998 | GFP (total IgY), chicken antibodyAS15 3002 | GFP (purified recombinant protein)other tag antibodies
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Recommended dilutions for use
1:250 - 1:500 (IC), 1:1000 - 1:2000 (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
mCherry
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
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References
to be added when available, antibody released in August 2016.
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Scientific context
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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