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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii Q93WD2
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS04 045 Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSIILHC available antibodies against pigment-binding proteinsCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:10 000 with standard ECL (WB)
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Molecular weight expected аpparent
29 for Chlamydomonas reinhardtii
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlamydomonas reinhardtii
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No reactivity
higher plants (use AS04 045 for those organsims), algae, cyanobacteria
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Supplementary information
to be added when available
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References
Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Drop et. al (2014). Consequences of state transitions on the structural and functional organization of Photosystem I in the green alga Chlamydomonas reinhardtii. Plant J. 2014 Feb 8. doi: 10.1111/tpj.12459.
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Scientific context
Lhcb4 (CP29) is a member of the family of chlorophyll a/b-binding proteins, which is conserved in higher plants and green algae. Lhcb4 is associated with Photosystem II serving both as a light-harvesting antenna protein as well as playing a role in photoprotective dissipation of excitation energy. The Chlamydomonas homolog of plant Lhcb4 has also been denoted as Lhcbm4.
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Notes
None
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Protein number
Q93WD2
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TAIR number
Refer to NCBI
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