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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide RKT*VAKPKGP, where T* indicates phospho-Thr
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 25 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding protein , rabbit antibodies AS13 2705 | Lhcb2 | LHCII type II chlorophyll a/b-binding protein, phosphorylated, rabbit antibodiesAll anti-LHC antibodiesAntibodies to other proteins involved in photosynthesisPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 10 000 with standard ECL (WB)
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Molecular weight expected аpparent
25 | 25 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Spinacia oleracea, Zea mays
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
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References
Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015. Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21. Jia et al. (2014). Accumulation of NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana. Plant J. 2014 Dec 30. doi: 10.1111/tpj.12753.Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.
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Scientific context
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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