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Available ordering format
Liquid in PBS pH 8.0, 0.02% sodium azide
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Immunogen
Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) P13853, At1g53540
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins
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Recommended dilutions for use
1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
17.6 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
n.a.
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No reactivity
no confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.
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References
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
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Scientific context
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
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Notes
total IgY concentration is 32.2 µg/µl
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Protein number
P13853
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TAIR number
AT1G53540
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