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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
Dot blot (Dot), chromatine immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Connected products
AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710
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Recommended dilutions for use
2-5 µg/milion cells (ChiP), 1: 1000 (Dot), 1: 50 (IF), 1: 50 (IHC), 1: 500 (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
human
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Possible reactivity
C.elegans, D. melanogaster, mouse, plant, rat, Xenopus sp.
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
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Notes
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
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Protein number
Q71DI3
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TAIR number
Refer to NCBI
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