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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
Dot blot (Dot), Western Blot (WB)
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Connected products
AS16 3182 | anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
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Recommended dilutions for use
1: 1000 (Dot), 1 µg/ml (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
human
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Possible reactivity
C.elegans, chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.
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Notes
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.01% sodium azide and 30% glycerol.
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Protein number
Q71DI3
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TAIR number
Refer to NCBI
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