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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana P46422, At4g02520
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), western blot (WB)
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Connected products
antibodies to plant endomembrane proteinsPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
24 | 26 kDa
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Verified reactivity
Arabidopsis thaliana, Liquidambar formosa, Schima superba
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Possible reactivity
dicots including: Brassica juncea
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufacture by Operon Biotechnologies.
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References
Datta and Chattopadhyay (2015). Changes in the proteome of pad2-1, a glutathione depleted Arabidopsis mutant, during Pseudomonas syringae infection. Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.
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Scientific context
Glutathione S-transferase PM24 (EC=2.5.1.18) is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
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Protein number
P46422
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TAIR number
AT4G02520
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