GSNO, Nitroso-L.glutathione
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Catalog number
AS08 361
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Price
Please ask
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Size
100 µl
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Available ordering format
Lyophilized present in PBS pH 7.35
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Immunogen
synthetic Nitroso-L.Glutathione conjugated to protein carrier (PC)
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Raised in
Rat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG purified by ammonium sulfate precipitation
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How to reconstitute
For reconstitution add 50 µl of distilled water and 50 µl of glycerol.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
immunocytochemistry (ICC), ELISA (ELISA)
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Connected products
no related to other products
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Recommended dilutions for use
1: 1000 - 1: 5000 (ICH), 1: 5000 - 1: 10 000 (ELISA), 1: 1000 (WB)
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Molecular weight expected аpparent
47 kDa
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Verified reactivity
GSNO nitroso-L.glutathione
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Possible reactivity
GSNO nitroso-L.glutathione
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
antibody has been used on plant tissue as well as on isolated arteries from a rat
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References
Chaki et al. (2009). Involvement of reactive nitrogen and oxygen species (RNS and ROS) in sunflower-mildew interaction. Plant Cell Physiol. 50:265-279. Corpas et al. (2008). Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants. Methods Enzymol. 437: 561-574.
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Scientific context
GSNO; S-nitrosoglutathione belongs to a family of molecules involved in nitrosative stress. GSNO is a natural source of nitric oxide (NO). S-nitrosylation reaction of NO with GSH leads to formation of S-nitroso glutathione (GSNO). Selected references: Barroso et al (2006). Localization of S-nitrosoglutathione and expression of S-nitrosoglutatione reductase in pea plants under cadmium stress. J Exp Bot. 2006;57(8):1785-93. Valderrama et al (2007). Nitrosative stress in plants. FEBS Lett. 6;581(3):453-6. Corpas et al (2008). Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants. Methods Enzymol. 437:561-74
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Notes
data on antibody specificity nad immunolocalization protocol can be found here
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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