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Available ordering format
Liquid
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Immunogen
Purified Human IgG, Fc fragment
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8 °C.
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Verified applications
This antibody is suitable for all immunoassay applications
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Connected products
other anti-human secondary antibodies
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Recommended dilutions for use
The optimal working dilution should be determined by the end user.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Human IgG Fc
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Human IgG Fc, min. cross-reactivity to bovine, horse, mouse or rabbit serum proteins or mouse IgG1 is a secondary antibody which binds to human IgG Fc in immunological assays.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - light chains on all human immunoglobulins - human IgA or IgM - serum proteins from bovine, horse, mouse, or rabbit .
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Protein number
O75015
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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Properties
Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Gene symbol
PGAP1, PGAP3, MPPE1
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Short name
Goat, IgM, , , mouse rabbit serum proteins
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Technique
Rabbit, Mouse, serum, Goat, IgM, goats, IgMs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgM, IgM
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Species
Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Mouses
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Alternative name
caprine, Immunoglobulin M, bovine, horse, mouse and production species: rabbit blood serum proteins
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Alternative technique
rabbit-anti, sera, murine, caprine, immunoglobulin-m
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Tissue
serum
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