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Available ordering format
Lyophilized
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Immunogen
Purified Human IgG, Fc fragment
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-human secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Human IgG Fc
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Human IgG Fc, DyLight® 680 conjugated, min. cross-reactivity to human IgA + IgM is a secondary antibody conjugated to DyLight® 680, which binds to human IgG Fc in immunological assays.DyLight® 680 has Amax = 682 nm, Emax = 715 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - light chains on all human immunoglobulins - human IgA or IgM .
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Protein number
O75015
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen