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Available ordering format
Lyophilized
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Immunogen
to be determined
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-rabbit secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Rabbit IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Rabbit IgG (H&L), DyLight® 405 conjugated, min. cross-reactivity to bovine, human, mouse IgG/serum is a secondary antibody conjugated to DyLight® 405, which binds to rabbit IgG in immunological assays. DyLight® 405 has Amax = 400 nm, Emax = 420 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - serum proteins from bovine, human, or mouse - IgG from human or mouse .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera