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Available ordering format
Lyophilized
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Immunogen
Purified Mouse IgG, whole molecule
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified Goat IgG
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How to reconstitute
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
Contact Gentaur support center
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Connected products
other dylight anti-mouse secondary antibodies
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Recommended dilutions for use
1:20 - 1:2,000 for most applications
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
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References
to be added when available
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Scientific context
Goat anti-mouse IgG (H&L) - DyLight® 550 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG (highly absorbed against rat IgG) is a secondary antibody conjugated to DyLight® 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
