Goat, Fc specific, Unconjugated
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Catalog number
AS16 3120
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Price
Please ask
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Size
1 ml
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Available ordering format
Inquire
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Immunogen
purified dog IgG from pooled dog serum
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Protein G purified IgG
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How to reconstitute
For reconstitution please add 1 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.
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Verified applications
immuniprecipitation (IP)
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Connected products
other unconjugated secondary antibodies to dog IgGSecondary antibodies
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Recommended dilutions for use
Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
dog IgG, Fc part
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Possible reactivity
to be determined
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No reactivity
The antiserum does not cross-react with any other component of the dog immunoglobulin system.
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Supplementary information
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details. No preservative added, as it may interfere with the antibody activity.
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References
to be added when available, antibody released in January 2016.
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Scientific context
Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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Conjugation
not conjugated
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Gene target
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Short name
Goat, Fc specific, Unconjugated
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Technique
Goat, FC, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
Unconjugated
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Species
Goats
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Alternative name
caprine, fragment c specific, nonconjugated
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Alternative technique
caprine
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