Goat, Fc, FITC conjugated, min.

• Catalog number
  AS16 3343
• Price
  Please ask
• Size
  1.0 mg

• Available ordering format
  Liquid
• Immunogen
  Purified Human IgG, Fc fragment
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store at 2-8 °C.
• Verified applications
  Flow cytometry (FACS), Immunomicroscopy (IM)
• Connected products
  other anti-human secondary antibodies
• Recommended dilutions for use
  1:20 - 1:2 000 (FACS and IM)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Human IgG Fc
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  To be added when available, antibody released in April 2016.
• Scientific context
  Goat anti-Human IgG Fc, FITC conjugated, min. cross-reactivity to bovine, horse, mouse or rabbit serum proteins or mouse IgG1 is a secondary antibody conjugated to FITC, which binds to human IgG Fc in immunological assays.
• Notes
  Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - light chains on all human immunoglobulins - human IgG F(ab)'2 fragment - mouse IgG1 - serum proteins from bovine, horse, mouse, or rabbit .
• Protein number
  O75015
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus

• Gene target
  conjugated   min  
• Short name
  Goat, Fc, FITC conjugated, min.
• Technique
  FITC, Goat, FC, antibody Conjugates, Fluorescein, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  FITC
• Species
  Goats
• Alternative name
  caprine, fragment c, fluorecein coupled, min.
• Alternative technique
  caprine, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products