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Available ordering format
Liquid
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Immunogen
Purified Rabbit IgG, whole molecule
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8 °C.
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Verified applications
This antibody is suitable for all immunoassay applications
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Connected products
Other anti-rabbit secondary antibodies
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Recommended dilutions for use
The optimal working dilution should be determined by the end user.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Rabbit IgG (H&L), F(ab)'2 Fragment
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment, min. cross-reactivity to non-immunoglobulin rabbit serum proteins and human serum proteins is a secondary antibody, which binds to rabbit IgG (H&L), F(ab)'2 Fragment in immunological assays.
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Notes
Antibody purity is > 90% based on SDS-PAGE .Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins .Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - serum proteins from human.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Short name
Goat, F(ab)'2 , min. cross-reactivity non-immunoglobulin rabbit serum proteins serum proteins
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Technique
Rabbit, serum, Goat, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Species
Human, Goats, Humans
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Alternative name
caprine, F(Antibody)'2 Fragment, min. cross-This (antibody to-) recognizes to non-immunoglobulin production species: rabbit blood serum proteins and H. sapiens blood serum proteins
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Alternative technique
rabbit-anti, sera, caprine
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Tissue
serum
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