Goat, F(ab)' fragment, ALP conjugated

  • Catalog number
    AS10 807
  • Price
    Please ask
  • Size
    0.5 mg
  • Available ordering format
    Liquid
  • Immunogen
    Purified human IgG
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified goat IgG
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
  • Connected products
    other secondary antibodies to human immunoglobulins
  • Recommended dilutions for use
    1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    human IgG Fc (two heavy chains with constant domains)
  • Possible reactivity
    human IgG Fc (two heavy chains with constant domains)
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.
  • References
    to be added when available
  • Scientific context
    Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
  • Notes
    ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservativePurity ≥ 90 % based on SDS-PAGE.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Latin name
    Capra aegagrus hircus
  • Gene target
    ALP   conjugated  
  • Gene symbol
    PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
  • Short name
    Goat, F(ab)' , ALP conjugated
  • Technique
    Goat, antibody Conjugates, goats
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
  • Species
    Goats
  • Alternative name
    caprine, F(Antibody)' fragment, ALP coupled
  • Alternative technique
    caprine
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