Goat, DyLight® 550 conjugated, min. cross-reactivity to human serum proteins

• Catalog number
  AS16 3516
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized
• Immunogen
  to be determined
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store freeze-dried powder at 2-8 °C.
• Verified applications
  Flow cytometry (FACS), Immunomicroscopy (IM)
• Connected products
  other anti-rabbit secondary antibodies
• Recommended dilutions for use
  1:20 - 1:2 000 (FACS and IM)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Rabbit IgG (H&L)
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  To be added when available, antibody released in April 2016.
• Scientific context
  Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated, min. cross-reactivity to human serum proteins is a secondary antibody conjugated to DyLight® 550, which binds to rabbit IgG in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
• Notes
  Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - human serum proteins .
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Capra aegagrus hircus
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Description
  Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Group
  Sera
• About
  Serum should be stored at +5°C

• Gene target
  DyLight®   550   conjugated   min   cross   proteins  
• Short name
  Goat, DyLight® 550 conjugated, min. cross-reactivity serum proteins
• Technique
  serum, Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  DyLight
• Species
  Goats, Humans
• Alternative name
  caprine, DyLight® 550 coupled, min. cross-This (antibody to-) recognizes to H. sapiens blood serum proteins
• Alternative technique
  sera, caprine
• Tissue
  serum

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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