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Available ordering format
Lyophilized
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Immunogen
Purified Human IgG, whole molecule
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-human secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Human IgG (H&L) F(ab)'2 Fragment
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Human IgG (H&L), F(ab)'2 Fragment, DyLight® 488 conjugated, min. cross-reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight® 488, which binds to human IgG (H&L), F(ab)'2 Fragment in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG - light chains on all human immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - serum proteins from bovine, mouse or rabbit .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Short name
Goat, DyLight® 488 conjugated, min. cross-reactivity , mouse, rabbit serum
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Technique
Rabbit, Mouse, serum, Goat, antibody Conjugates, goats, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Label
DyLight
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Species
Bos Taurus genes are available form the Bovine Genome database. Goats, Mouses
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Alternative name
caprine, DyLight® 488 coupled, min. cross-This (antibody to-) recognizes to bovine, mouse, production species: rabbit blood serum
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Alternative technique
rabbit-anti, sera, murine, caprine
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Tissue
serum
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