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Available ordering format
Lyophilized
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Immunogen
Purified Mouse IgG, whole molecule
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-mouse secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Mouse IgG (H&L), F(ab)'2 Fragment
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment, DyLight® 350 conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight® 350, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays. DyLight® 350 has Amax = 353 nm, Emax = 432 nm.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on mouse IgG - light chains on all mouse immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin mouse serum immunoglobulins - serum proteins from bovine, horse, human rabbit or swine .
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Protein number
AAA51107
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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Properties
Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Short name
Goat, DyLight® 350 conjugated, min. cross-reactivity , , , rabbit serum proteins
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Technique
Rabbit, serum, Goat, antibody Conjugates, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Label
DyLight
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Species
Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Humans, Pigs and Guinea pigs
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Alternative name
caprine, DyLight® 350 coupled, min. cross-This (antibody to-) recognizes to bovine, horse, H. sapiens, swine and production species: rabbit blood serum proteins
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Alternative technique
rabbit-anti, sera, caprine
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Tissue
serum
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