GDP, Galactose Phosphorylase
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Catalog number
AS10 723
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Price
Please ask
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Size
200 µl
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Available ordering format
Lyophilized
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Immunogen
KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
Plant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1200 with alkaline phosphatase, 1: 3000 with regular ECL (WB)
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Molecular weight expected аpparent
51 | 50 kDa
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Verified reactivity
Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
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Possible reactivity
dictos: Sorghum bicolor, monocots: Oryza sativa, moss: Physcomitrella patens
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
to be added when available, antibody available in July 2010
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Scientific context
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
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Notes
None
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Protein number
Q8LKQ7
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TAIR number
AT4G26850
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Gene target
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Short name
GDP, Galactose Phosphorylase
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Alternative name
GDP, Galactose Phosphorylase
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