GDE / AGL, Glycogen debranching enzyme
#
-
Catalog numberAS09 454
-
Price:
-
Size200 µg
-
-
Available ordering formatLyophilized
-
ImmunogenKLH-conjugated peptide derived from the sequence of human glycogen debranching enzyme P35573
-
Raised inRabbit
-
ClonalityPolyclonal
-
ClonePolyclonal
-
PurificationAffinity purified serum
-
How to reconstituteFor reconstitution add 200 µl of sterile water
-
Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applicationswestern blot (WB), immunoprecipitation (IP)
-
Connected productsAS09 455 | anti-glycogen phosphorylaseSecondary antibodies
-
Recommended dilutions for use1: 2000 (WB), 5 µg (IP)
-
Molecular weight expected аpparent175 kDa
-
Verified reactivityhuman, mouse, rabbit,rat
-
Possible reactivitydog
-
No reactivityno confirmed exceptions from predicted reactivity known in the moment
-
Supplementary informationsamples used to test this antibody were: muscle and liver homogenates, purified glycogen
-
ReferencesOldenburg et al. (2016). CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer. 2016 Sep 5;16:713. doi: 10.1186/s12885-016-2756-5.Pagliarani et al. (2014). Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Aug 1. pii: S0925-4439(14)00250-6. doi: 10.1016/j.bbadis.2014.07.029.
-
Scientific contextSynonymes:Glycogen debrancher, 4-alpha-glucanotransferase (EC=2.4.1.25), Oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-1,6-glucosidase (EC=3.2.1.33), Dextrin 6-alpha-D-glucosidase,amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
-
NotesThis antibody can detect GDE in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GDE.Antibody will work as an excellent marker for glycogen. In tissues with no glycogen extression there will be no signal using this antibody.
-
Protein numberP35573
-
TAIR numberRefer to NCBI
-
DescriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
-
Gene target
-
Gene symbolAGL
-
Short nameGDE / AGL, Glycogen debranching enzyme
-
TechniqueEnzyme, enzymes
-
Alternative nameGDE / AGL, Glycogen debranching enzyme
-
Alternative techniqueenzymes
-
Gene info
-
Identity
-
Gene
-
Long gene nameamylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
-
Synonyms gene name
- amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1992-07-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Glycoside hydrolases
-
VEGA ID