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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
collection of antibodies to plant stress proteins
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Recommended dilutions for use
1: 5000 with standard ECL (WB), 1: 5000 (IL)
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Molecular weight expected аpparent
50 kDa
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Verified reactivity
Arabidopsis thaliana, Nicotiana tabcum, Solanum lycopersicum, Zea mays
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Possible reactivity
dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
antibodies has been used in immunolocalization on Arabidopsis thaliana
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References
Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.Ghanta et al. (2011). Nicotiana tabacum overexpressing gamma-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.
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Scientific context
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.
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Notes
total IgG concentration is 5.2 µg/ µl
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Protein number
Q8W4W3
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TAIR number
AT4G23100
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