FITC, Fluoresceine isothiocyanate

• Catalog number
  AS16 3978
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  BSA-conjugated synthetic FITC.
• Raised in
  N/A
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified by Ammonium Sulfate Precipitation.
• How to reconstitute
  For reconstitution add 50 µl of sterile water and 50 µl of glycerol. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Stable for two years at 4°C. For long time storage, aliquot and store product at -20°C. Avoid cycles of freezing and thawing.
• Verified applications
  ELISA, Immunocytochemistry, Immunohistochemistry (IHC), Immunoprecipitation (IP), in situ hybridization (ISH)
• Connected products
  no related to other products
• Recommended dilutions for use
  To be determined by end user.
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Conjugated Fluoresceine (FITC).
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  This antibody has been pre-adsorbed.
• References
  to be added when available, product available since September 2016.
• Scientific context
  Fluoresceine isothiocyanate (FITC) is an amine reactive fluorenscence label, used in many applications. This antibody recognizes conjugated FITC and the free Fluoresceion molecule, and it can be used for amplifying weak signals in immunofluorescent detection.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Fluoresceine   isothiocyanate  
• Short name
  FITC, Fluoresceine isothiocyanate
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  fluorecein, Fluoresceine isothiocyanate
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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