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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 126 Fer1 | ferritin 1(pre-apoferritin)Collection of antibodies to Chlamydomonas proteins
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Recommended dilutions for use
0.736111111111111
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Molecular weight expected аpparent
32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlamydomonas reinhardtii
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
a picture with a western blot application example can be found in the reference given below
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References
Long et al. (2008) Genetics 179: 137-147
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Scientific context
Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.
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Notes
None
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Protein number
A8WCT5
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TAIR number
Refer to NCBI
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