Donkey, L), ALP conjugated

• Catalog number
  AS09 628
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Liquid
• Immunogen
  Purified sheep IgG, whole molecule
• Raised in
  Donkey
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified donkey IgG
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
• Connected products
  no related to other products
• Recommended dilutions for use
  1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  sheep IgG heavy and light chains (H&L)
• Possible reactivity
  sheep IgG heavy and light chains (H&L)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
• References
  to be added when available
• Scientific context
  Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
• Notes
  APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Equus africanus asinus

• Gene target
  ALP   conjugated  
• Gene symbol
  PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
• Short name
  , L), ALP conjugated
• Technique
  antibody Conjugates
• Host
  Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Species
  Donkeys
• Alternative name
  Donkey, L), ALP coupled

Gene info

• Identity
  HGNC:20767
• Gene
  PDLIM3
• Long gene name
  PDZ and LIM domain 3
• Synonyms
  • ALP
• GenBank acession
  • AF002280
• Locus
  4q35.1
• Discovery year
  2004-02-06
• Entrez gene record
  27295
• Pubmed identfication
  • 10063829
  • 8828038
• RefSeq identity
  • NM_014476
• Classification
  • PDZ domain containing
  • LIM domain containing
• VEGA ID
  OTTHUMG00000160412
• Locus Specific Databases
  • LRG_752

Gene info

• Identity
  HGNC:16448
• Gene
  ASRGL1
• Long gene name
  asparaginase and isoaspartyl peptidase 1
• Synonyms gene name
  • asparaginase like 1
• Synonyms
  • FLJ22316
  • ALP1
  • ALP
• Synonyms name
  • asparaginase-like 1 protein
• Locus
  11q12.3
• Discovery year
  2001-08-29
• Entrez gene record
  80150
• Pubmed identfication
  • 11984834
  • 28671681
  • 27106100
• RefSeq identity
  • NM_001083926
• VEGA ID
  OTTHUMG00000167513

Gene info

• Identity
  HGNC:29063
• Gene
  ATRNL1
• Long gene name
  attractin like 1
• Synonyms gene name
  • attractin-like 1
• Synonyms
  • KIAA0534
  • FLJ45344
  • ALP
• GenBank acession
  • AB011106
• Locus
  10q25.3
• Discovery year
  2004-03-04
• Entrez gene record
  26033
• Pubmed identfication
  • 9628581
• RefSeq identity
  • NM_207303
• Classification
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000019096

Gene info

• Identity
  HGNC:11092
• Gene
  SLPI
• Long gene name
  secretory leukocyte peptidase inhibitor
• Synonyms gene name
  • secretory leukocyte protease inhibitor (antileukoproteinase)
• Synonyms
  • HUSI-I
  • ALK1
  • ALP
  • BLPI
  • HUSI
  • WAP4
  • WFDC4
• Synonyms name
  • antileukoproteinase
• GenBank acession
  • X04502
• Locus
  20q13.12
• Discovery year
  1998-04-24
• Entrez gene record
  6590
• Pubmed identfication
  • 3640338
  • 12206714
• Classification
  • WAP four-disulfide core domain containing
• VEGA ID
  OTTHUMG00000033075

Gene info

• Identity
  HGNC:10626
• Gene
  CCL27
• Long gene name
  C-C motif chemokine ligand 27
• Synonyms gene
  • SCYA27
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 27
  • chemokine (C-C motif) ligand 27
• Synonyms
  • ALP
  • ILC
  • CTACK
  • skinkine
  • ESkine
  • PESKY
  • CTAK
• Synonyms name
  • CC chemokine ILC
  • IL-11 Ralpha-locus chemokine
  • cutaneous T-cell attracting chemokine
• GenBank acession
  • AJ243542
• Locus
  9p13.3
• Discovery year
  1999-07-30
• Entrez gene record
  10850
• Pubmed identfication
  • 10556532
  • 10588729
• RefSeq identity
  • NM_006664
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000019834

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