Donkey, IgG

  • Catalog number
    AS16 3511
  • Price
    Please ask
  • Size
    1 mg
  • Available ordering format
    Lyophilized
  • Immunogen
    to be determined
  • Raised in
    Donkey
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store freeze-dried powder at 2-8 °C.
  • Verified applications
    Flow cytometry (FACS), Immunomicroscopy (IM)
  • Connected products
    other anti-rabbit secondary antibodies
  • Recommended dilutions for use
    1:20 - 1:2 000 (FACS and IM)
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    Rabbit IgG (H&L)
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    To be added when available, antibody released in April 2016.
  • Scientific context
    Donkey anti-Rabbit IgG (H&L), DyLight® 405 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight® 405, which binds to rabbit IgG in immunological assays. DyLight® 405 has Amax = 400 nm, Emax = 420 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries
  • Notes
    Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep .
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Latin name
    Equus africanus asinus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Short name
    , IgG
  • Technique
    IgG, IgGs
  • Host
    Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Isotype
    IgG, IgG
  • Species
    Donkeys
  • Alternative name
    Donkey, Immunoglobulin G
  • Alternative technique
    immunoglobulins
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