Donkey, DyLight® 488 conjugated
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Catalog number
AS16 3526
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Price
Please ask
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Size
0.5 mg
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Available ordering format
Lyophilized
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Immunogen
Purified Rabbit IgG, whole molecule
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Raised in
Donkey
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
Other anti-rabbit secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Rabbit IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLight® 488 conjugated is a secondary antibody conjugated to DyLight® 488, which binds to rabbit IgG in immunological assays. DyLight® 488 has Amax = 493 nm, Emax = 518 nm. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Equus africanus asinus
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Gene target
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Short name
, DyLight® 488 conjugated
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Technique
antibody Conjugates
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Host
Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Label
DyLight
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Species
Donkeys
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Alternative name
Donkey, DyLight® 488 coupled
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