Donkey, DyLight® 488 conjugated
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Catalog number
AS10 843
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Price
Please ask
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Size
1 mg
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Available ordering format
Lyophilized
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Immunogen
Purified Rabbit IgG, whole molecule
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Raised in
Donkey
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified donkey IgG
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How to reconstitute
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
Contact Gentaur support center
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Connected products
other DyLight® anti-Rabbit secondary antibodies
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Recommended dilutions for use
1:20 - 1:2,000 for most applications
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available
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Scientific context
Donkey anti-Rabbit IgG (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Equus africanus asinus
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Gene target
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Short name
, DyLight® 488 conjugated
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Technique
antibody Conjugates
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Host
Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Label
DyLight
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Species
Donkeys
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Alternative name
Donkey, DyLight® 488 coupled
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