CO4, Complement component C4, Biotin conjugated
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Catalog number
IMS06-032-304
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Price
Please ask
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Size
100 µl
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Available ordering format
Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
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Immunogen
full length native protein P0C0L4 purified from human plasma
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
IMS02-032-303 | anti-Complement component C4IMS01-031-305 | anti-Complement component C4cIMS01-066-309 | anti-Complement factor H
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Recommended dilutions for use
use at an assay dependent dilution (WB)
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Molecular weight expected аpparent
193 kDa
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Verified reactivity
human
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Possible reactivity
human
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum or human C4. Only one precipitin arc in immunoelectrophoresis against normal human serum or human C4. Specific against human C4 alpha and beta chains in electroblotting when tested against normal human serum.
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References
to be added when available
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Scientific context
Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
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Notes
affinity purified on human C4 agaroseconcentration: 1 mg/ml
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Protein number
P0C0L4
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene target
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Gene symbol
C4B, C4A
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Short name
CO4, Complement component C4, conjugated
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Technique
antibody Conjugates
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Label
Biotin
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Alternative name
CO4, Complement component C4, biotinilated coupled
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Gene info
Gene info
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