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Available ordering format
Liquid
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Immunogen
to be determined
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Raised in
Chicken
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
Contact Gentaur support center
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Connected products
other ALP conjugated secondary antibodies to goat IgG
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Recommended dilutions for use
1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG/serum and are affinity purified using solid phase goat IgG.
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Notes
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse,rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
