Chicken, IgG or serum proteins
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Catalog numberAS12 1973
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PricePlease ask
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Size0.5 mg
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Available ordering formatLyophilized
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ImmunogenPurified rat IgG
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Raised inChicken
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified chicken IgG
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How to reconstituteFor reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage conditionStore lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applicationsContact Gentaur support center
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Connected productsother dylight anti-rat secondary antibodies
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Recommended dilutions for use1:20 - 1:2,000 for most applications
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Molecular weight expected аpparentsee datasheet
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Verified reactivityto be determined
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Possible reactivityto be determined
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No reactivityto be determined
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Supplementary informationBased on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from human or rabbit serum proteins from human or rabbit.
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Referencesto be added when available
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Scientific contextChicken anti-Rat IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to DyLight® 550, which binds to all rat IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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NotesConjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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PropertiesChickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
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DescriptionAzide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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GroupSera
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Gene target
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Gene symbolPGAP1, PGAP3, MPPE1
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Short name, IgG serum proteins
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Techniqueserum, IgG, IgGs
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IsotypeIgG, IgG
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Specieschicken, Chickens
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Alternative nameChicken, Immunoglobulin G and blood serum proteins
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Alternative techniquesera, immunoglobulins
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Tissueserum
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Gene info
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Identity
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Gene
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Long gene namepost-GPI attachment to proteins inositol deacylase 1
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Synonyms gene name
- post-GPI attachment to proteins 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2008-02-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepost-GPI attachment to proteins phospholipase 3
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Synonyms gene
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Synonyms gene name
- per1-like domain containing 1
- post-GPI attachment to proteins 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-04-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemetallophosphoesterase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data