-
Available ordering format
Lyophilized
-
Immunogen
Purified Rabbit IgG
-
Raised in
Chicken
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified Chicken IgY
-
How to reconstitute
For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
-
Storage condition
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
-
Verified applications
ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
-
Connected products
other antibodies to rabbit IgG HRP conjugated
-
Recommended dilutions for use
optimal working dilution should be determined by the investigator
-
Molecular weight expected аpparent
see datasheet
-
Verified reactivity
rabbit IgG (H&L)
-
Possible reactivity
rabbit IgG (H&L)
-
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
-
Supplementary information
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.
-
References
to be added when available
-
Scientific context
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
-
Notes
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
