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Available ordering format
Liquid
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Immunogen
to be determined
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Raised in
Chicken
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
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Verified applications
Contact Gentaur support center
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Connected products
other FITC conjugated secondary antibodies to rabbit IgG
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Recommended dilutions for use
1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Chicken anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rabbit IgG.
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Notes
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
