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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP), immunolocalization (IL)
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Connected products
AS06 184 | anti-AO | apoplastic ascorbate oxidaseAS09 384 | anti-AO | ascorbate oxidaseAS09 383 | anti-AO | ascorbate oxidase, biotinylated antibodyAS08 368 | anti-APX | ascorbate peroxidasePlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:10 000 with standard ECL (WB), 2 µg (IP), 1: 500 (IL)
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Molecular weight expected аpparent
28 kDa
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Verified reactivity
Arabidopsis thaliana (immunohistochemistry only) D. salina, A. toxicaria, Hordeum vulgare, Pisum sativum, Silene vulgaris, Solanum lycopersicum, Solanum lycopersicum, Solanum tuberosum, Picea silvestris, Zea mays
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Possible reactivity
Brassica. juncea, Citrus sinensis, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum, Solanum tuberosum, Sorghum bicolor, Vitis vinifera, trees: Pinus pinaster
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No reactivity
Glycine max
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Supplementary information
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References
Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015. Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814. Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)
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Scientific context
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
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Notes
total IgG concentration is 3 µg/µl
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Protein number
Q41772
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TAIR number
ATCG00350
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
