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Available ordering format
Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
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Immunogen
full length native protein P02769
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB), immunodifusion (ID)
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Connected products
AS99 002 | anti-BSA rabbit antibodyAS02 023 | anti-BSA hen antibodyIMS06-035-327 | anti-BSA hen antibody, biotinylatedSecondary antibodies
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Recommended dilutions for use
1: 5 000 with standard ECL (WB)
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Molecular weight expected аpparent
67 kDa
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Verified reactivity
bovine
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Possible reactivity
goat, sheep
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
only one precipitin arc in immunodiffusion against normal bovine serum, dilution 1:100, or bovine albumin. Only one precipitin arc in immunoelectrophoresis against normal bovine serum or bovine serum albumin.
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References
to be added when available
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Scientific context
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids and thyroid hormones, and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
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Notes
affinity purified IgY on bovine serum albumin agarose
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Protein number
P02769
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TAIR number
Refer to NCBI
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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About
Serum should be stored at +5°C