-
Available ordering format
Lyophilized
-
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified serum
-
How to reconstitute
For reconstitution add 50 µl of sterile water
-
Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
ELISA (ELISA), western blot (WB), immunofluorescence (IF), immunogold (IG)
-
Connected products
AS09 615 | BiP2 | lumenal-binding protein 2, goat antibodyAS09 614 | BiP2 | lumenal-binding protein 2, hen antibodyAS09 481PRE | BiP | lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
-
Recommended dilutions for use
1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 600 (IF)
-
Molecular weight expected аpparent
73.5 | 80 kDa
-
Verified reactivity
dicots: Arabidopsis thaliana, Brassica napus, Cucumis sativus, Nicotiana benthamiana, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, monocots: Triticum aestivum, Zeay mays, trees: Picea abies, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
-
Possible reactivity
Capsella rubella, Vitis vinifera, Ricinus comminus, Glycine max, Citrus clementina, Citrus sinsensis, Prunus persica, Arabis alpina, Isatis tincorina, Eucalyptus grandis, Capsicum annuum, Triticum aestivium. dicots including: Nicotiana tabacum, monocots: Hordeum vulgare, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa.
-
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
-
Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.
-
References
Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715 Botella et al. (2015). ALA10, a phospholipid flippase, controls FAD2/FAD3 desaturation of phosphatidylcholine in the ER, and affects chloroplast lipid composition in Arabidopsis thaliana. Plant Physiol. 2015 Nov 30. pii: pp.01557.2015. Hu et al. (2015). Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Jan 12. pii: pp.114.252023. Ivanov et al. (2014). SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1. Plant Cell. 2014 Mar 4. Bommert et al. (2013). The maize Gα gene COMPACT PLANT2 functions in CLAVATA signalling to control shoot meristem size. Nature,Sep 11. doi: 10.1038/nature12583. (western blot, Zea mays) Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization).
-
Scientific context
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
-
Notes
Method for plant ER isolation is available here.
-
Protein number
Q39043
-
TAIR number
AT5G42020, AT5G42020
-
