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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
immunofluorescence (IF), Western blot (WB), Blue Native-PAGE (BN-PAGE)
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Connected products
AS05 085-10 | anti-AtpB rabbit antibody, smaller pack size of AS05 085AS05 085PRE | AtpB | beta subunit of ATP synthase, pre-immune serumAS03 030 | anti-AtpB hen antibody (developed to exactly the same peptide as rabbit antibody)AS03 030S | ATP synthase subunit beta protein standard for quantitation and positive controlAS08 304 | anti-ATP synthase subunit alpha antibodyAS08 312 | anti-ATP synthase subunit gamma antibodyAS05 071 | anti-ATP synthase subunit c antibodyrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:100 (IF), 1: 2000 - 1: 5 000 with standard ECL (WB), 1: 5000 (BN-PAGE)
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Molecular weight expected аpparent
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
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Verified reactivity
Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, E.coli, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp. (roots, leafs, pollen), Nicotiana bentamiana, Nicotiana tabacum, Pheodactylum tricornutum CCAP 1055/1, Plasmodium berghei, Populus sp., Selaginella martensii, Spinacia oleracea, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria
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Possible reactivity
Acinetobacter baumannii, algae, Clostridium sp., cyanobacteria, E.coli K-12, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Yrsinia sp.
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No reactivity
archeal V-type ATP synthase
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Supplementary information
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
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References
Jallet et al. (2016). Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime. Algal research, doi:10.1016/j.algal.2016.05.014. Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205. Shah and Duncan (2015). Aerobic growth of Escherichia coli is reduced and ATP synthesis is selectively inhibited when five C-terminal residues are deleted from the ϵ subunit of ATP synthase. J Biol Chem. 2015 Jul 9. pii: jbc.M115.665059. Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.Teng et al. (2013). Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase. PLOS ONE, June 2013. (immuofluorescence)Rasala et al. (2013). Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. March 23.Heinnickel et al. (2013). Novel thylakoid membrane greencut protein cpld38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes. J. Biol. Chem. Jan 9.
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Scientific context
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
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Notes
the anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii
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Protein number
P19366 , P83483 , P06541 , A8IQU3
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TAIR number
AT5G08670, ATCG00480
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