ATPase, ATP synthase, whole enzyme

• Catalog number
  AS08 370
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  Purified native protein from spinach
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water. To get the best possible dissolution please keep it at +4°C ON.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  AS08 304 | anti-ATP synthase subunit alpha antibodyAS03 030 | anti-ATP synthase subunit beta hen antibodyAS08 312 | anti-ATP synthase subunit gamma antibodyAS05 071 | anti-ATP synthase subunit c antibodyAS12 1853 | anti-ATP synthase subunit c antibodyPlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 10 000 (WB)
• Molecular weight expected аpparent
  38-58 kDa
• Verified reactivity
  subunits alpha, beta, gamma, epsilon of Ch. reinhardtii, S. oleracea, P.sativum, perennial grasses: Eremochloa ophiuriudes, Paspalum vaginatum
• Possible reactivity
  n. a.
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  the prominent reaction is to a doublet around 55-58 kDa which is alpha and beta and weaker reaction to gamma subunit which is around 38 kDa
• References
  Liu et al. (2011). Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance. J Plant Physiol. Nov 7
• Scientific context
  ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

• Gene target
  ATPase   ATP   synthase   whole  
• Gene symbol
  VPS9D1, NFATC2
• Short name
  ATPase, ATP synthase, whole enzyme
• Technique
  Enzyme, enzymes
• Alternative name
  ATPase, adenosine triphosphate synthase, whole enzyme
• Alternative technique
  enzymes
• Tissue
  whole

Gene info

• Identity
  HGNC:13526
• Gene
  VPS9D1
• Long gene name
  VPS9 domain containing 1
• Synonyms gene
  • C16orf7
• Synonyms gene name
  • chromosome 16 open reading frame 7
• Synonyms
  • ATP-BL
• GenBank acession
  • AB018551
• Locus
  16q24.3
• Discovery year
  2000-09-20
• Entrez gene record
  9605
• Pubmed identfication
  • 10231027
• RefSeq identity
  • NM_004913
• Classification
  • VPS9 domain containing
• VEGA ID
  OTTHUMG00000173219

Gene info

• Identity
  HGNC:7776
• Gene
  NFATC2
• Long gene name
  nuclear factor of activated T cells 2
• Synonyms gene name
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T-cells 2
• Synonyms
  • NF-ATP
  • NFATp
  • NFAT1
• GenBank acession
  • U43342
  • U43341
• Locus
  20q13.2
• Discovery year
  1994-11-16
• Entrez gene record
  4773
• Pubmed identfication
  • 8202141
• RefSeq identity
  • NM_012340
• Classification
  • IPT domain containing
  • Nuclear factors of activated T-cells
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032747

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