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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCaP1 Q9SXE9, At1g62480
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA)
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Connected products
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 with standard ECL (WB), 1: 8000 (ELISA)
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Molecular weight expected аpparent
16.6 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manuafactured by Operon Biotechnologies.
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References
Ide et al. (2007). Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark. Plant Cell Physiol. 1:54-65.
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Scientific context
CCaP1 acts as a mediator in response to continuous dark or gibberellic acid. Alternative name: At1g62480/T3P18_4, T3P18.4
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plant vacuolar membranes can be found here.
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Protein number
Q9SXE9
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TAIR number
AT1G62480
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