ASyO2 | Mouse anti-human alpha-synuclein, oligomer-specific
#
-
Catalog numberAS13 2717
-
Price:
-
Size50 µg
-
-
Available ordering formatLyophilized
-
Immunogensynthetic peptide derived from human alpha-synuclein Glu131-Ala140
-
Raised inMouse
-
ClonalityMonoclonal
-
CloneIgG1, (clone number 51.24)
-
PurificationAffinity purified
-
How to reconstituteFor reconstitution add 50 µl of sterile water.
-
Storage conditionFor short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applicationsdot blot (Dot), ELISA (ELISA), immunolocalization (IL)
-
Connected productsAS13 2718 | anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodiesAS13 2719 | anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodiesAS13 2717B | anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodiesAS13 2718B | anti-ASyO5-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodiesAS13 2719B | anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodiesAS08 358 | anti-SNCA | Alpha-synuclein, rabbit antibodiesSecondary antibodiesprotein extraction buffer Secondary antibodies
-
Recommended dilutions for use2-4 ug/ml (ELISA capture), 10 ug/ml (IHC), 1-2 ug/ml (Dot)
-
Molecular weight expected аpparent14 kDa
-
Verified reactivityhuman
-
Possible reactivitymouse
-
No reactivityno confirmed exceptions from predicted reactivity are currently known
-
Supplementary informationto be added when available
-
ReferencesBrännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
-
Scientific contextAlpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
-
NotesNone
-
Protein numberRefer to NCBI
-
TAIR numberRefer to NCBI
-
DescriptionThe ASyO2 | Mouse anti- alpha-synuclein, oligomer-specific is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Testagisera supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Latin nameMus musculus
-
Gene target
-
Short nameASyO2 | Mouse anti- alpha-synuclein, oligomer-specific
-
Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, mouses
-
Hostmouse
-
SpeciesHuman, Humans, Mouses
-
Alternative nameASyO2 | Mouse Antibody tohuman a-synuclein, oligomer-specific
-
Alternative techniquemurine, antibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data