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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A UniProt: Q39219, TAIR: At3g22370, AOX1B UniProt: O23913, TAIR:AT3G22360, AOX1C UniProt: O22048, TAIR: AT3G27620, and AOX2, UniProt: O22049, TAIR: AT5G64210, Solanum lycopersicum UniProt: Q7XBG9, Oryza sativa UniProt: Q7XT33, AOX1D, TAIR: AT1G32350
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western Blot (WB), Immunolocalization (IL)
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Connected products
AS06 152 AOX1 | alternative oxidase from Chlamydomonas reinhardtii, rabbit antibodyAS10 699 AOX | alternative oxidase monoclonal antibodyAS04 054PRE | AOX1/2 | plant alternative oxidase 1 and 2, pre-immune serumAS04 054S | AOX | AOX positive control/quantitation standardRecommended secondary antibody (for western blot with ECL detection)Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection (WB), 1: 750 (IL)
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Molecular weight expected аpparent
36-40 | 36-40 for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Solanum tuberosum, Solanum esculentum, Physcomitrella patens
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Possible reactivity
Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Nicotiana benthamiana, Oryza brachyantha, Oryza sativa, Populus tremula, Picea sitchensis, Triticum aestivum, Saccharum officinarum, S.officinarumea, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Zea mays, Vigna unguiculata, Vitis vinifera
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.Contains 0.01% proclin
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References
Zhao et al. (2016). Nitrogen deprivation induces cross-tolerance of Poa annua callus to salt stress. Biologia Plantarum 60 (3): 543-554.Solti et al. (2016). Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake? Planta. 2016 Dec;244(6):1303-1313. Epub 2016 Aug 19.Zhang et al. (2016). A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice (Oryza sativa L.). PLoS Genet. 2016 Jul 1;12(7):e1006152. doi: 10.1371/journal.pgen.1006152. eCollection 2016.Meng et al. (2016). Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep. 2016 Mar 15;6:23098. doi: 10.1038/srep23098Pavlovic et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Konert et al.(2015). Protein phosphatase 2A (PP2A) regulatory subunit B'gamma interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana. New Phytol. 2015 Feb;205(3):1250-63. doi: 10.1111/nph.13097. Epub 2014 Oct 13.Wang et al. (2015). Proteomic analysis of changes in the Kandelia candel chloroplast proteins reveals pathways associated with salt tolerance. Plant Sci. 2015 Feb;231:159-72. doi: 10.1016/j.plantsci.2014.11.013. Epub 2014 Dec 4.Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.Tsaniklidis et al. (2014). Study of alternative oxidase in seeded and parthenocarpic cherry tomato fruits during their development and postharvest storage. Acta Physiologiae Plantarum, September 2014. (immunolocalization)Zubo et al. (2014). Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria. Mitochondrion. 2014 Mar 31. pii: S1567-7249(14)00037-3. doi: 10.1016/j.mito.2014.03.011
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Scientific context
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
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Notes
Mitochondrion inner membrane marker. Possibly in the inner surgace of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here.
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Protein number
Q39219 , O22049 , Q7XBG9 , Q7XT33
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TAIR number
AT3G22370, AT5G64210
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
