Alkaline, lipase (glyoxysomal lipase)
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Catalog number
AS10 698
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Price
Please ask
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Size
100 µl
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Available ordering format
Lyophilized
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Immunogen
native, purified alkaline lipase from Ricinus communis
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
antibodies to proteins involved in plant lipid metabolismPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 2000 with standard ECL (WB)
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Molecular weight expected аpparent
62 kDa
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Verified reactivity
Coffea arabica, Ricinus communis
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Possible reactivity
higher plants
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Manufactured by Operon Biotechnologies.
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References
Patui et al. (2014). Lipase activity and antioxidant capacity in coffee (Coffea arabica L.) seeds during germination. Plant Sci. 2014 Apr;219-220:19-25. doi: 10.1016/j.plantsci.2013.12.014. Epub 2014 Jan 3.
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Scientific context
Alkaline lipase is a glyoxysomal enzyme which breaks down fat molecules. Endogenous storge lipid in Ricinus communis, tricinolein is hydrolyzed by this enzyme, localized in the glyoxysomes of endosperm.
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Notes
Method for preparation of plant glyoxysomal membrane.Serum contains less than 0.1 % of sodium azide as preservative.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Gene target
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Short name
Alkaline, lipase (glyoxysomal lipase)
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Alternative name
Alkaline, lipase (glyoxysomal lipase)
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