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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AKIN10 sequence Q38997, At3g01090
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl/tube of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodyAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodyAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodyAS09 463 | Anti-AKIN beta gamma, rabbit antibodyAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antiboddyAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodycollection of antibodies to proteins involved in signal transductionPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 500 with standard ECL (WB)
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Molecular weight expected аpparent
61.1 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
Oryza sativa, Solanum lycopersicum, Vitis vinifera
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Supplementary information
AKIN10 antibody works best when first the SnRK1 complex is immunorecipitated with this or another antibody and then anti-AKIN10 antibody is used to probe with. When this antibody is used against a lysate, the dominant rubisco protein can mask any immunoreactivity.
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References
Nukarinen et al. (2016). Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Sci Rep. 2016 Aug 22;6:31697. doi: 10.1038/srep31697.Castro et al. (2015). SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signalling and Metabolism in Arabidopsis thaliana. Plant Cell Physiol. 2015 Oct 14. pii: pcv149.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Rodrigues et al. (2013). ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis. Plant Cell, Oct 31.
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Scientific context
AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2
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Notes
None
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Protein number
Q38997
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TAIR number
AT3G01090
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Description
The AKIN10, SNF1- protein kinase catalytic subunit alpha KIN10 is a α- or alpha protein sometimes glycoprotein present in blood.
