Agrisera, IncuBlocker (rabbit, 2x20 ml trial pack)
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Available ordering format
Inquire
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Immunogen
to be determined
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Raised in
N/A
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Clonality
N/A
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Clone
N/A
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at +4°C.
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Verified applications
Contact Gentaur support center
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Connected products
Larger Pack Size: AS16 3111 | Agrisera IncuBlocker (rabbit, 2x100 ml)AS16 3111-1L | Agrisera IncuBlocker (rabbit, 2x1L)IncuBlocker for secondary antibodies from other species:Agrisera IncuBlocker (chicken)Agrisera IncuBlocker (mouse)
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Recommended dilutions for use
see datasheet
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Easy - Quick - ClearAgrisera IncuBlocker (rabbit) is an "all in one" kit for fast western blot processing. Whole process takes place in a single step as blocking, primary and secondary antibody binding occur simultaneously. Followed by a wash step with included buffer. Depending upon titer of primary antibody it can be re-used up to 5 times (not recommended for quantification work). The kit contains all you need to get you started, except for primary antibody of your choice and chemiluminescence developing reagent, which you can also purchase from Agrisera: AgriseraECL BrightEnhanced (highest sensitivity).Frequently Asked Questions about IncuBlocker are answered here.
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Notes
Kit components: Wash Buffer (Reagent A) and the "all in one" goat anti-rabbit HRP-conjugated secondary antibody in advanced blocking solution (Reagent B).Required: primary antibody of your choice and chemiluminescense detection kit, for example Agrisera ECL BrightEnhanced.Assay time: 15 minutes to 1 hour + 3 x 5 minutes washProcedure: Washing (3 x 5 min) --> Incubation with IncuBlocker (15 min-1h) --> Washing (3-4 x 5 min)Instruction of use. Step by step:1. Add 2 ml of a Wash Buffer (Reagent A), shake at RT for 5 min. then discard the wash buffer. Repeat this step 3 times.2. Add your primary antibody to 20 ml of reagent B (recommended concentration 0.2-0.5 µg/ml for affinity purified antibodies or standard dilution recommended by manufacture for antibodies in format of serum.3. Add this solution to your membrane and shake at RT for 15 min-1h (optimized by end user).4. Discard or collect the solution then return to step 1. 5. Following this procedure your membrane ready for detection using chemiluminescence.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Agrisera, IncuBlocker (rabbit, 2x20 trial pack)
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Technique
Rabbit
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Host
Rabbit, Rabbits
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Alternative name
Agrisera, IncuBlocker (production species: rabbit, 2x20 milliliter trial pack)
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Alternative technique
rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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